WebThe antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these … WebPurified anti-ATF4 Antibody. Total cell lysates (15 µg protein) from non-treated and Tunicamycin (Tm) treated (2 µg/mL, six hours) HeLa and 293T were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-ATF4 (clone W16016A) antibody or competitor's antibody used at the ...
ATF4 Polyclonal Antibody (10835-1-AP) - Thermo Fisher Scientific
WebDec 19, 2024 · Shipping Temperature : Blue Ice or 4ºC. Cite this Product : StressMarq Biosciences Cat# SMC-447D, RRID: AB_2701793. Certificate of Analysis : 1 µg/ml of SMC-447 was sufficient for detection of ATF4 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. WebMar 25, 2024 · Western Blot analysis of Rat Brain showing detection of ~39 kDa (isoform 2) ATF4 protein using Mouse Anti-ATF4 Monoclonal Antibody, Clone S360A-24 (56583). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ATF4 Monoclonal Antibody (56583) at … great ice cream toppings
Purified anti-ATF4 Antibody anti-ATF4 - W16016A - BioLegend
WebNov 1, 2024 · Membranes were then incubated at 4 °C overnight with ATF4 antibody (BioLegend) at 1:1000. After washing with 1x TBST, anti-rat HRP secondary antibody (CST) was used at 1:2000. A list of commercially available antibodies tested in livers and cell lines is included in Supplementary Table 1. 2.6. Gene expression analysis. WebDec 17, 2024 · BackgroundThe role of activating transcription factor 4 (ATF4) underlying gastric cancer (GC) remains unclear. The purpose of this study was to investigate the expression levels and biological functions of ATF4 in GC.MethodsExpression of ATF4 was detected by quantitative PCR (qPCR), Western blotting, and immunohistochemistry. Cox … WebOverview Product name Anti-ATF-4 antibody See all ATF-4 primary antibodies Description Rabbit polyclonal to ATF-4 Host species Rabbit … floating homes for sale seattle wa